Translational_Unit

Part:BBa_K4213037:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-03)


TetO7-p35S-AtPHT1;5 non opt-Venus-tHSP.tNOS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal EcoRI site found at 2963
    Illegal PstI site found at 347
    Illegal PstI site found at 4058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal EcoRI site found at 2963
    Illegal PstI site found at 347
    Illegal PstI site found at 4058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal EcoRI site found at 2963
    Illegal BglII site found at 3992
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal EcoRI site found at 2963
    Illegal PstI site found at 347
    Illegal PstI site found at 4058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal EcoRI site found at 2963
    Illegal PstI site found at 347
    Illegal PstI site found at 4058
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GoldenBraid 2.0


Source

-

References